Cerebrospinal fluid biomarkers for the differential diagnosis of normal pressure hydrocephalus and Alzheimer's disease
Item
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Title
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Cerebrospinal fluid biomarkers for the differential diagnosis of normal pressure hydrocephalus and Alzheimer's disease
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Identifier
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d_2009_2013:86f88836a951:10534
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identifier
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10830
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Creator
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Assuras, Stephanie,
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Contributor
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Lisa D. Ravdin | Joan C. Borod
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Date
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2010
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Language
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English
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Publisher
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City University of New York.
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Subject
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Psychobiology | Neurobiology | Alzheimer's Disease | Biomarkers | Dementia | Normal Pressure Hydrocephalus | Proteomics
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Abstract
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Differential diagnosis of Idiopathic Normal Pressure Hydrocephalus (NPH) is complicated by symptomatic overlap with neurodegenerative conditions such as Alzheimer's Disease (AD). Efforts to improve diagnosis through the use of cerebrospinal fluid (CSF) biomarkers have led to the identification of more than a dozen potential diagnostic markers for NPH. However, no single biomarker has proven sufficient for differential diagnosis in clinical practice. The current study uses proteomic analysis of CSF to identify sets of protein markers that are expressed differentially in NPH and AD. Two-dimensional gel electrophoresis was used to analyze the CSF of 8 probable NPH and 8 probable AD patients. Gels were stained with SYPRO Ruby and the percentage volume of over 1339 spots was determined. The Random Forest statistical method was used to identify proteins that optimally segregated NPH cases from AD. Protein identification was achieved by the use of a previously published CSF map and mass spectrometry. Eleven protein spots were found to optimally distinguish the groups, correctly classifying 100% of all NPH and AD samples. Of the 11 proteins of interest, six were identified and include the following: beta-trace, serum albumin A, serum albumin B, apolipoprotein A-IV precursor, pigment epithelium-derived factor, and complement component 3 precursor. The current study identifies CSF biomarkers that differentiate between NPH and AD cases. The highly successful separation of cases obtained in this study suggests that multiplexed CSF markers have the potential to improve the differential diagnosis of NPH from one of its most common competing diagnoses.
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Type
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dissertation
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Source
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2009_2013.csv
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degree
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Ph.D.
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Program
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Psychology
