Synthesis, Characterization and Applications of Microsphere-Supported Biomembranes
Item
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Title
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Synthesis, Characterization and Applications of Microsphere-Supported Biomembranes
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Identifier
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d_2009_2013:4d4431b125d1:10890
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identifier
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11209
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Creator
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He, Bin,
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Contributor
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M. Lane Gilchrist
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Date
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2011
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemical engineering | Materials science | Biomembranes | FRAP | Scaffold | Supported Lipid Bilayer
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Abstract
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Our objective is to develop a microsphere-based platform for assaying the function, interactions, and inhibition of membrane proteins. To functionalize such materials with active membrane proteins, the challenge is to build stabilized, microsphere-supported biomembranes in which the substrate to biomembrane spacing can be controlled to accommodate larger membrane proteins. These systems are termed proteolipobeads or lipobeads depending on the constituents.;The silica microsphere (4.7 micron) surface was functionalized with DiNHS-PEG in order to make a tethering bridge designed to yield greater spacing. Confocal fluorescence microscopy was utilized to analyze the silica surface at different stages of surface modification and examine the passivation of the substrate and the formation of lipid bilayers. The lateral mobility and fluidity of the supported membranes was analyzed using fluorescence recovery after photobleaching (FRAP). Flow cytonmetry and Fluorescence Activated Cell Sorting (FACS) was used as a biomembrane quality control measure, an important advantage of the lipobead format.;These structures have been employed as a route to functionally immobilize the yeast drug efflux pump PDR5, an important anti-fungal drug target. Another intention is to develop a new platform to present molecules to stem cells within a biomimetic architecture: as laterally-mobile molecules embedded in the context of a tailored biomembrane.
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Type
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dissertation
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Source
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2009_2013.csv
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degree
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Ph.D.
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Program
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Engineering
