Genetic variance contributes to opioid and dopamine receptor modulation of sucrose and fat intake and sucrose-conditioned preferences in inbred mouse strains
Item
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Title
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Genetic variance contributes to opioid and dopamine receptor modulation of sucrose and fat intake and sucrose-conditioned preferences in inbred mouse strains
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Identifier
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d_2009_2013:e36f0b4014d2:11539
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identifier
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12072
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Creator
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Dym, Cheryl T.,
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Contributor
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Richard J. Bodnar
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Date
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2012
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Language
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English
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Publisher
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City University of New York.
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Subject
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Physiological psychology | Psychobiology | Neurosciences | Conditioned Preferences | Dopamine | Feeding | Inbred Mouse Strains | Opioids
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Abstract
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Whereas genetics and pharmacology influence nutrient consumption, the current dissertation used inbred mouse strains to examine genetic variance in the dopaminergic and opioid modulation of sucrose and fat. The first and second specific aims measured intake of a sucrose solution in 8 inbred and 1 outbred mouse strains following administration of opioid (naltrexone (NTX)), dopamine D1 (SCH23390), and D2 (raclopride) receptor antagonists. NTX inhibited intake strongly in C57BL/10 and C57BL/6, moderately in BALB/cJ, C3H/He, CD-1 and DBA/2, weakly in 129P3 and SJL/J, and not at all in the SWR/J mouse strains. SCH23390 attenuated sucrose intake across five (129P3/J, SJL/J), four (C57BL/6J, BALB/cJ), three (SWR/J, C3H/HeJ, C57BL/10J, DBA/2J) and two (CD-1) of the doses tested. Raclopride was wholly ineffective in attenuating intake. In the third specific aim, intake of a fat solution (Intralipid) was measured in 8 out of 9 prior strains following NTX and SCH23390 administration. NTX attenuated intake at four (DBA/2), three (SWR/J, SJL/J), two (CD-1, C57BL/10), one (C57BL/6, 129P3) and none (BALB/cJ) of the doses tested. SCH23390 reduced intake at five (DBA/2, SWR/J, CD-1), four (SJL, C57BL/6), three (129P3), one (C57BL/10) and none (BALB/cJ) of the doses tested. A high correlation was found in the strain-dependent abilities of SCH23390 and NTX to suppress Intralipid, but not sucrose intake, suggesting differential pharmacological mechanisms responsible. The fourth specific aim investigated genetic variance in experiential factors by examining whether SCH23390 and NTX alter acquisition and expression of a sucrose-conditioned flavor preference (CFP) in BALB/cJ and SWR/J inbred mouse strains. Mice received either vehicle, SCH23390 or NTX prior to acquisition: alternate daily exposure to a sucrose solution mixed with one flavor (CS+/S) and saccharin solution mixed with another flavor (CS-/s) or expression: a two-bottle choice test with the two flavors mixed in saccharin. In expression, strong CS+ preferences were reduced by SCH in BALB and SWR mice and by NTX in SWR mice. In acquisition, CS+/S was reduced by SCH in both strains, and by NTX in BALB/cJ. Sucrose-CFP was reduced by NTX BALB/cJ mice and SCH in SWR/J mice. Taken together, future studies are needed to reconcile the divergent results between strains, pharmacological systems, and nutrients to fully understand their influence on nutrient consumption and CFP.
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Type
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dissertation
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Source
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2009_2013.csv
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degree
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Ph.D.
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Program
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Psychology
