The expression of the transcription factor broad and RNA-binding factors in the midgut of the mosquito Aedes aegypti
Item
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Title
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The expression of the transcription factor broad and RNA-binding factors in the midgut of the mosquito Aedes aegypti
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Identifier
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d_2009_2013:11f2ad89b0b8:12074
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identifier
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12730
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Creator
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Ray, Kathryn,
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Contributor
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James T. Nishiura
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Date
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2013
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Language
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English
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Publisher
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City University of New York.
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Subject
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Molecular biology | Aedes aegypti | BROAD (BR) | JH | midgut | miRNA | mosquito development
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Abstract
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Transcription factors, microRNAs and RNA binding factors frequently interact to coordinate gene expression during development. The transcription factor BROAD (BR) is a global regulator of insect gene transcription and governs the timing of the commitment to pupate. I determined BR expression in the Ae. aegypti midgut by qPCR, and correlated its expression with that of nine miRNAs and three RNA-binding factors. During midgut metamorphosis the expression of these factors was dynamic and reproducible.;To better understand the changes in expression patterns, I evaluated the effects of hormone analogs on expression. Using this approach I uncovered concurrent up-regulation of BR, miR-34and miR-14 in the pupal midgut when treated with methoprene, and found that RH2485 accelerated expression of BR, BRAT, and microRNAs let-7 and miR-125. Treatment with each hormone analog resulted in a change in BR expression.;Finally, I evaluated the effect of nutrients on expression levels. Surprisingly, though most transcripts were down-regulated during starvation, the expression of BR did not decrease, while microRNAs miR-34 and miR-14 were elevated. This may identify a novel role for miR-34 during starvation in an invertebrate, and raises the possibility that miR-34 and miR-14 are part of a starvation-induced stress response in the mosquito midgut.;In summary, this expression analysis suggests that microRNA regulation plays an important role during midgut metamorphosis, and reveals a new layer of regulatory complexity in the control of development in Ae. aegypti .
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Type
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dissertation
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Source
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2009_2013.csv
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degree
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Ph.D.
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Program
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Biology
