Raman-microscopy of individual cells and applications monitoring drug uptake.
Item
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Title
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Raman-microscopy of individual cells and applications monitoring drug uptake.
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Identifier
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AAI3205013
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identifier
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3205013
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Creator
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Matthaeus, Christian B.
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Contributor
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Adviser: Max Diem
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Date
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2006
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Physical | Chemistry, Pharmaceutical
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Abstract
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Raman imaging of individual cells has become a quite useful technique providing spectral information at very reasonable signal quality. Based on the spectral parameters of a cell's components it is possible to image subcellular compartments such as nucleus or nucleoli at micrometer spatial resolution. Apparent advantages over conventional staining procedures are the noninvasive character of the technique as well as the associated chemical information. Depending on the optical setup Raman microscopy can be employed after cell fixation or on living cells. One intriguing application of Raman microscopy is monitoring the uptake of cytotoxins that are for example used in chemotherapy. The spectral information allows imaging the subcellular distribution of the drug as well as speculation about its chemical faith based on spectral changes. In chapter 3, the capability of Raman microscopy to image subcellular compartments is demonstrated. First by univariate intensity imaging, followed by introducing spectral imaging based on multivaried statistical clustering analysis. Chapter 4 addresses the potential of the technique to study drug uptake in in vitro type cell cultures.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
