Induced optical fluorescence spectroscopy of laser excited microorganisms in water.

Title: Induced optical fluorescence spectroscopy of laser excited microorganisms in water.
Identifier: AAI3024820
identifier: 3024820
Creator: Musa, Sarhan Mahmoud.
Contributor: Adviser: Samir A. Ahmed
Date: 2001
Language: English
Publisher: City University of New York.
Subject: Engineering, Electronics and Electrical | Biology, Microbiology
Abstract: This thesis presents a study of the laser induced optical natural fluorescence of Giardia lamblia cysts. It shows that the auto-fluorescence spectra of untagged Giardia lamblia cysts excited by visible lasers can be detected using fiber-optical fluorescence microscopy. The peak of the fluorescence spectrum agrees with the amino acid tryptophan in aqueous solution at all the excitation wavelengths used. While G. lamblia's fluorescence is very similar to other translucent microorganisms such as paramecium and rotifer under 458 nm excitation, a clear difference is revealed under blue laser excitation at 401 nm, where only G. lamblia matches tryptophan in fluorescence. A reproducible decay of the fluorescence intensity is also observed in Giardia lamblia under continuous excitation.
Type: dissertation
Source: PQT Legacy CUNY.xlsx
degree: Ph.D.

Media: Induced optical fluorescence spectroscopy of laser excited microorganisms in water.