p53-TFIID-DNA interaction: Analysis by DNA affinity chromatography.
Item
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Title
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p53-TFIID-DNA interaction: Analysis by DNA affinity chromatography.
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Identifier
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AAI3037424
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identifier
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3037424
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Creator
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Molina, Maria Patricia.
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Contributor
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Adviser: Jill Bargonetti
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Date
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2002
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry | Biology, Molecular
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Abstract
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Previous immunoprecipitation experiments have shown that p53 has the ability to associate with the TATA binding protein and some of its associated factors, and that this association modulates p53 function. By DNA affinity chromatography we studied the binding of wild-type p53 and the mentioned proteins to the p53-binding sites present in the Promoter 2 (P2) of the MDM2 gene, in the Ribosomal Gene Cluster (RGC) and to the ideal p53-binding site called superconsensus sequence (SCS). We report that p53 can be purified by DNA affinity chromatography using the above-mentioned p53-binding sites. We also report that the p53-binding sites select for specific p53 subpopulations, which have different binding characteristics, "bindomers". Our results demonstrate that DNA affinity Chromatography is a promising technique to isolate these p53 subpopulations or ("bindomers") in order to perform further biochemical analysis. We also show that the mdm2 P2 binding p53 is able to induce the addition of other(s) factor(s) to a TBP-TAFII60-TAFII40 complex. We show that other forms of wt p53 do not induce this addition.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
