BRAIN CLATHRIN: ULTRASTRUCTURAL, BIOCHEMICAL AND IMMUNOLOGICAL STUDIES.
Item
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Title
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BRAIN CLATHRIN: ULTRASTRUCTURAL, BIOCHEMICAL AND IMMUNOLOGICAL STUDIES.
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Identifier
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AAI8023690
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identifier
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8023690
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Creator
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BLOOM, WILLIAM SAMPSON.
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Date
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1980
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, General
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Abstract
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Among the many cellular events awaiting elucidation, endocytosis is the process whose molecular nature is least understood. Morphologically, a coated vesicle is the organelle believed to play a pivotal role in endocytosis; an advance in understanding endocytosis could be accomplished if coated vesicles were fractionated, the coat components separated, the proteins identified and properties characterized. At the time this study was undertaken, steps in the enrichment of coated vesicles and identification of their components had been reported. It was felt, therefore, that the isolation and characterization of clathrin--the major element of the coat--was the logical next step and an attainable goal. Clathrin was purified to a high degree and its ultrastructural features analyzed. The finding that clathrin formed baskets or cages in vitro convinced researchers that clathrin is the main structural protein of the coat. The next step was to specifically localize clathrin in cells. Its antigenicity permitted antibodies to be prepared and purified. In this way, clathrin was visualized in cells as lattices giving a distinct pattern. The fact that antibodies to brain clathrin recognized clathrin from other cells suggested that it is phylogenetically well preserved, widespread, and essential for cell functions.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biomedical Sciences
