EFFECT OF 3,4-DIHYDROXYBUTYL-1-PHOSPHONATE ON PHOSPHOGLYCERIDE AND LIPOTEICHOIC ACID METABOLISM IN BACILLUS SUBTILIS.
Item
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Title
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EFFECT OF 3,4-DIHYDROXYBUTYL-1-PHOSPHONATE ON PHOSPHOGLYCERIDE AND LIPOTEICHOIC ACID METABOLISM IN BACILLUS SUBTILIS.
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Identifier
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AAI8023697
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identifier
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8023697
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Creator
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DEUTSCH, ROBERT M.
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Contributor
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Burton E. Tropp
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Date
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1980
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry
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Abstract
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3,4-Dihydroxybutyl-1-phosphonate (CH2CHOHCH20HCH2PO3H), an analog of glycerol 3-phosphate preferentially inhibits the rate of synthesis and accumulation of phosphatidylglycerol in Bacillus subtilis 1005 and BD170. The rate of phosphatidylethanolamine synthesis is only slightly inhibited whereas that of lysylphosphatidylglycerol is somewhat stimulated. As expected, decreased phosphatidylglycerol synthesis results in the inhibition of the formation of the putative lipoteichoic acid precursor, sn-glycero-1-phospho-B-gentiobiosyldiacylglycerol and of lipoteichoic acid itself in strain 1005. Turnover of phospholipids was not affected by drug. Accumulation of phosphatidylglycerol and lysylphosphatidylglycerol was depressed in the presence of drug which follows from synthesis and turnover studies. Neutral lipids accumulated in the presence of drug. Upon removal of drug a striking enhancement of the synthesis of phosphatidylglycerol and lysylphosphatidylglycerol as compared to synthesis in untreated cultures occurred. Phosphatidylethanolamine synthesis remained normal. The 'stimulatory' effect on lipid synthesis that occurred after removing the drug was greater in strain 1005 than in strain BD170. On the other hand, all areas of synthetic activity in the presence of drug were inhibited to a greater extent in Strain BD170 than in strain 1005. The ability of bacteriophage 025 to bind to its cell wall receptor in Strain BD170 is decreased dramatically after a 60 minute incubation with drug.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biochemistry
