THE EFFECT OF DIFFERENT ALGAL PROTEIN CONCENTRATIONS ON THE GROWTH, PROTEIN CONVERSION, AND NITROGEN BALANCE OF JUVENILE MERCENARIA CAMPECHIENSIS, THE SOUTHERN HARD CLAM.
Item
-
Title
-
THE EFFECT OF DIFFERENT ALGAL PROTEIN CONCENTRATIONS ON THE GROWTH, PROTEIN CONVERSION, AND NITROGEN BALANCE OF JUVENILE MERCENARIA CAMPECHIENSIS, THE SOUTHERN HARD CLAM.
-
Identifier
-
AAI8023704
-
identifier
-
8023704
-
Creator
-
GOLDSTEIN, BARRY B.
-
Contributor
-
John H. Tietjen
-
Date
-
1980
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Biology, Oceanography
-
Abstract
-
A Tahitian strain of Isochrysis sp. was grown in outdoor continuous culture and fed at four different cell densities to juveniles of the Southern hard clam, Mercenaria campechiensis. These cell densities were: 1 x 10('4), 5 x 10('4), 1 x 10('5), and 5 x 10('5) cells/ml. Controls consisted of trays without animals receiving an inflow cell density of 5 x 10('4) cells/ml and trays with animals, but receiving only filtered seawater. Each treatment went to duplicate populations of 100 animals; each population had a whole wet weight of 10 grams. The total flow rate to each population was 120 ml per minute.;The incoming filtered seawater, incoming algal culture, and effluent from each shellfish population was collected daily to be analyzed for nitrite, nitrate, ammonia, urea, dissolved free amino acids (DFAA), soluble protein (SP), total dissolved nitrogen (TDN), and particulate protein nitrogen (PPN).;A nitrogen balance for juvenile Mercenaria campechiensis in a continuous flow system was calculated; 85 to 95 percent of all total incoming nitrogen was accounted for in the different treatments.;The pattern of change in concentration of the various nitrogen containing compounds as a result of the passage through the shellfish culture containers is described. There was a significant excretion of ammonia only by those populations receiving an inflow algal protein concentration (APC) of 5.75 (mu)gat. PPN/1. If there was any excretion of DFAA or urea, there was an equal uptake of these compounds by microorganisms present in the shellfish culture containers. There was a significant uptake of both nitrite and nitrate by the algae present in the copious biodeposits of those shellfish populations receiving an inflow APC of 56.01 (mu)gat. PPN/1. There was a significant uptake of soluble protein by those shellfish populations receiving (GREATERTHEQ) 5.75 (mu)gat. PPN/1.;Protein Stripping Efficiency, Protein Retention Efficiency, Food Chain Efficiency, Ecological Efficiency, Protein Conversion Efficiency, and Gross Growth Efficiency were all maximal for those animals receiving an average weekly inflow APC of 5.75 (mu)gat. PPN/1, which corresponds to an ambient APC of 2.93 (mu)gat. PPN/1.;Ingested ration increased with increasing ambient APC; this does not conform to current models of food concentration and ingestion.;Weekly biomass production, regardless of how measured, was maximal for those animals receiving the intermediate inflow APC of 5.75 and 11.33 (mu)gat. PPN/1. There was no significant difference at the 95% confidence level in weekly biomass production between those clam populations receiving these intermediate inflow APC. There was less growth exhibited by those clam populations receiving higher or lower inflow algal protein concentrations. These results do not conform to current models of bivalve mollusc feeding and growth.;An inflow algal protein concentration of 5.75 (mu)gat. PPN/1 flowing at a rate of 120 ml/min. to 10 grams live weight of juvenile Mercenaria campechiensis contained in 250 ml of seawater results in an ambient algal protein concentration of 2.93 (mu)gat. PPN/1. It is concluded that this ambient algal protein concentration is optimum for the growth and protein conversion efficiency of juvenile Mercenaria campechiensis.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
-
Program
-
Biology
