Applications of capillary electrophoresis.
Item
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Title
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Applications of capillary electrophoresis.
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Identifier
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AAI3063897
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identifier
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3063897
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Creator
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Wu, Hua (Angela).
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Contributor
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Adviser: David C. Locke
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Date
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2002
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Analytical
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Abstract
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Part I. Determination of capsaicins in capsicum annuum fruits using micellar electrokinetic capillary chromatography. A simple, rapid method for the quantitative determination of capsaicins using micellar electrokinetic capillary chromatography (MECC) was optimized, validated, and applied to several varieties of Capsicum fruits. Capsaicins are extracted using acetonitrile (ACN). The injection sample was prepared by diluting the extraction or standard stock solution in 10mM sodium dodecyl sulfate (SDS) aqueous solution. The best working condition includes a background electrolyte (BGE) containing 40mM SDS, 20mM Tris buffer, and 10% ACN (v/v) at pH 9.0; an UV detector at 280nm, and a 70cm x 50mum (50cm effective length) fused silica capillary operated at 30kV and 30°C.;Part II. Study of DNA-ruthenium(II) complex binding using capillary zone electrophoresis. Capillary zone electrophoresis (CZE) was utilized in this study to investigate the binding of a group of Ruthenium(II)-alpha-diimine complexes to calf thymus DNA. The chiral complexes were resolved due to their differential interaction with DNA in the separation medium. Buffer composition was optimized in terms of DNA concentration and salt concentration. The CZE enantioseparation with DNA as the chiral selector allowed a rapid screening for both stereoselectivities and relative binding constants with DNA. The actual binding constants were determined using a frontal analysis method.;Part III. Evaluation of association constant between basic enantiomers and sulfated-beta-cyclodextrin using a counter-current partial filling technique . Enantioseparations based on a counter-current mode were obtained as the analytes passed through the zone of BGE containing sulfated cyclodextrin, which moved towards the injection end and interacted with it transiently and differentially. The aim of this study was to evaluate association constants using the linearity between the apparent migration time and the partial filling fraction according to a proposed theoretical model. The experimental results showed that the apparent migration time does not change linearly with the partial filling fraction. The nonlinearity was explained by field strength effect.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
