FACTORS WHICH MAY INFLUENCE THE DETECTED FREQUENCY OF ANTIGENIC VARIANTS BY MONOCLONAL ANTIBODIES AND IDIOTYPY OF CLONAL RESPONSES TO INFLUENZA VIRUS HEMAGGLUTININ.
Item
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Title
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FACTORS WHICH MAY INFLUENCE THE DETECTED FREQUENCY OF ANTIGENIC VARIANTS BY MONOCLONAL ANTIBODIES AND IDIOTYPY OF CLONAL RESPONSES TO INFLUENZA VIRUS HEMAGGLUTININ.
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Identifier
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AAI8319779
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identifier
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8319779
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Creator
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LIU, YUNG-NAN CHIA.
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Contributor
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Jerome L. Schulman | Terry A. Krulwich
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Date
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1983
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology
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Abstract
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The studies involved in my research consisted of two aspects:;(I) Factors which may influence the frequencies of antigenic variants detected by monoclonal antibodies. The frequencies of antigenic variants detected by monoclonal antibodies against the same or overlapping antigenic determinants on the hemagglutinin of influenza B/Lee/40 virus were found to vary from 10('-5.5) to < 10('-8.1). These differences are related to differences in the avidities of the monoclonal antibodies used in selection: namely, there is an inverse relationship between antibody avidity and detected frequency of antigenic variants. When monoclonal antibodies against X31 hemagglutinin were employed in the studies, several observations were made: (a) an inverse relationship between antibody avidity and detected frequencies of antigenic variants was found using a single monoclonal antibody (X146) and different viruses, (b) the concentration of antibody used in variant selection affected the detected frequency of antigenic variants at least in some instances, (c) different frequencies of antigenic variants were detected by monoclonal antibody XY103 when different assay systems were employed.;(II) Idiotype of clonal responses to influenza virus hemagglutinin. Extensive cross-reactive idiotypy was demonstrated among monoclonal antibodies to distinct antigenic determinants on PR8 hemagglutinin. Analysis of idiotypes on monoclonal antibodies to B/Lee HA revealed: (a) a true individual idiotype not shared by any other monoclonal antibody, (b) idiotypic determinants shared by a few monoclonal antibodies, and (c) cross-reactive idiotypes which could be detected on all of the monoclonal antibodies tested. In addition, when the idiotypes expressed during primary and secondary responses of BALB/c mice immunized with influenza B/Lee virus were examined, it was found that some cross-reactive idiotypes were expressed during primary and secondary responses whereas others were detected only in the primary or secondary response.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biomedical Sciences
