Involvement of MAP kinase cascade in EMF induced signal transduction pathways.
Item
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Title
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Involvement of MAP kinase cascade in EMF induced signal transduction pathways.
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Identifier
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AAI3074672
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identifier
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3074672
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Creator
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Nie, Kui.
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Contributor
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Adviser: Ann Henderson
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Date
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2003
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Cell
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Abstract
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We have reported previously that differentiation of HL-60 human leukemia cells from a nonphagocytic suspension culture to an attached fibroblast-like culture with high phagocytic activity elicited by 12-O-tetradecanoylphorbol-13-acetate (TPA) could be induced by exposure to a 60 Hz, 1 G electromagnetic field (EMF). In this study, the impacts of EMF on MAP kinase pathway that is know to be involved in TPA induction were investigated in this cell line. We found that a small but significant increase in the level of activation of two members of MAPK kinase (MAPK) cascade, Raf-1 and MAP kinase, could be seen in these cells after they were exposed to the field for a short time (less than 30 minutes). Similar effects of EMF were also registered in MCF-7 human breast cancer cells and rat fibroblast 3Y1 cells. The levels of Raf-1 and MAPK activation in EMF exposed cells are equivalent to those observed in cells treated with low concentration of TPA (0.05--1 ng/ml). The involvement of PKC in the process leading to activation of MAPK cascade in cells exposed to EMF is also implicated by the present results. MAPK activation is inhibited by an inhibitor to PKC alpha, but not PKC delta inhibitors, in cells subjected to EMF exposure or TPA treatment. Thus, similarities between the effects of EMF and TPA treatment are supported by this investigation. This provides a possible method for revealing other participants in EMF-cell interaction, since the TPA induction pathway is well documented.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
