THE METABOLIC EFFECTS AND ILLICIT TRANSPORT OF 3,4-DIHYDROXYBUTYL-1-PHOSPHONATE IN ESCHERICHIA COLI.
Item
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Title
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THE METABOLIC EFFECTS AND ILLICIT TRANSPORT OF 3,4-DIHYDROXYBUTYL-1-PHOSPHONATE IN ESCHERICHIA COLI.
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Identifier
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AAI8401925
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identifier
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8401925
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Creator
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CONNOLLY, MARY MARGARET.
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Contributor
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Burton Tropp
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Date
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1983
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology
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Abstract
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3,4-Dihydroxybutyl-1-phosphonate (DHBP), an inhibitor of phosphoglyceride synthesis, exerts a bacteriostatic effect on the growth of Escherichia coli. Inhibition of growth by the analog is lessened or offset in strains having a large glycerol-3-phosphate pool. The effect of 3,4-dihydroxybutyl-1-phosphonate on growth was also examined in conjunction with deoxycholate, a bile salt present in the intestine, the natural habitat of the organism. The DHBP and deoxycholate together, exert a bactericidal effect on growth as determined by viability studies. This synergistic effect of the analog and deoxycholate also appears to depend on the intracellular glycerol-3-phosphate pool.;DHBP was also examined for its in vivo effect on the fatty acyl CoA: sn-glycerol-3-phosphate acyltransferase. When assayed for acyltransferase activity, membrane preparations isolated from DHBP treated cells have approximately the same Km value (8.4 x 10-5 M) as those isolated from untreated cells (9.5 x 10-5 M).;The metabolism of DHBP labeled with tritium in the 1,2 position was studied. Chromatographic analysis of either the formic acid soluble or water soluble cell fractions reveals the presence of a metabolite of DHBP, as yet unidentified.;The oligopeptide transport system in E. coli was studied for possible exploitation as a means of phosphonic acid analog uptake. DHBP as well as the phosphonic acid analogs of dihydroxyacetone phosphate and phosphomevalonic acid were coupled to several different tripeptides and their effect on the growth of E. coli investigated. The analogs of dihydroxyacetone phosphate and phosphomevalonate do not appear to be transported in the strains tested unless coupled to peptides in which case they inhibit growth. These results indicate possible transport of the phosphonate coupled peptides via the oligopeptide transport system.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biochemistry
