GENETIC ANALYSIS OF THE TRANSFORMED PHENOTYPE IN MOUSE CELLS (ONCOGENES, DNA TRANSFER, CARCINOGENESIS).
Item
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Title
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GENETIC ANALYSIS OF THE TRANSFORMED PHENOTYPE IN MOUSE CELLS (ONCOGENES, DNA TRANSFER, CARCINOGENESIS).
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Identifier
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AAI8423104
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identifier
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8423104
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Creator
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SMALL, MICHAEL BARRY.
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Contributor
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Harvey Ozer
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Date
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1984
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology
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Abstract
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Several lines of evidence, in particular that derived from study of the molecular biology of the acute transforming retroviruses, has suggested that there exist specific cellular genes whose aberrant expression leads to neoplastic cellular growth. Furthermore, it has been hypothesized that these genetic loci are the putative targets for somatic mutation by the action of carcinogenic agents. In an effort to identify such cellular "transforming genes" in mouse fibroblasts transformed in vitro by treatment with chemical carcinogens, the technique of DNA-mediated gene transfer was employed; more specifically, cellular DNA from chemically transformed mouse cells, co-precipitated into normal recipient cells. Expression of donor transforming genes was selected for by isolation of cells which have lost their dependence for anchorage as a requirement for cell growth (i.e. cells which can grow in semi-solid medium).;The normal mouse fibroblast cell line, NIH 3T3, was used as the recipient for DNA-mediated gene transfer. Attempts to use normal diploid human fibroblasts as DNA recipients proved not to be fruitful; however, in the course of such studies, a cloned derivative of the papovavirus, SV40, defective in autonomous replication, was found to be exceptionally efficient at transforming human fibroblasts.;Transformation activity was identified in a number of DNA's isolated from independent chemically transformed mouse cells. Two different cell lines were chosen for additional characterization. One, a nitrosocarbaryl-transformed 3T3 cell line, proved to harbor an exogenous Kirsten murine sarcoma virus genome. The other, a dimethylbenzanthracene-transformed 3T3 cell line, appears to have an activated cellular transforming gene which is homologous to the oncogene of Kirsten murine sarcoma virus.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biochemistry
