A PHOTOIONIZATION DETECTOR FOR HPLC USING A MICROPROCESSOR - CONTROLLED INTERFACE.
Item
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Title
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A PHOTOIONIZATION DETECTOR FOR HPLC USING A MICROPROCESSOR - CONTROLLED INTERFACE.
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Identifier
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AAI8501171
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identifier
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8501171
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Creator
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SCHMERMUND, JOHN THOMAS.
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Contributor
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David C. Locke
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Date
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1984
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Analytical
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Abstract
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The purpose of this investigation was to develop a highly sensitive and selective detector for high performance liquid chromatography (HPLC) and detect trace levels of pharmaceuticals in animal feed.;The initial study included the design of a high output vacuum ultraviolet lamp (VUV) and the construction of an ion chamber to collect the photoionized species formed after total vaporization of the column effluent.;Although the initial data was promising, limitations in the selection of aqueous mobile phases was noted due to the quenching of photoionized species by the vaporized mobile phase.;A microprocessor-controlled system was developed which could be used for complex, even thermally unstable mixtures separated by any mode of HPLC. The system described in this thesis has the flexibility to be used with both normal and reverse-phase LC with only minor modifications.;The new system was applied to the trace level analysis of pharmaceuticals spiked in animal feed. Excellent results were achieved for the separation of phenobarbital with a detection level of 30 ng. In addition, amantadine used as an antiviral, could be separated and detected on a reverse-phase system down to 250 ng.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Chemistry
