REGULATION OF RAT MEGAKARYOCYTE DEVELOPMENT IN PLASMA CLOT CULTURE SYSTEM.
Item
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Title
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REGULATION OF RAT MEGAKARYOCYTE DEVELOPMENT IN PLASMA CLOT CULTURE SYSTEM.
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Identifier
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AAI8508741
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identifier
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8508741
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Creator
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UYAR, RUHI.
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Contributor
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G. W. Cooper
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Date
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1985
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Animal Physiology
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Abstract
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An improved plasma clot culture system was developed for the growth of rat megakaryocytic cells. These cells were characterized histochemically by an acetyl-cholinesterase positive (AChE+) staining reaction. The conditioned medium, obtained from pokeweed-mitogen stimulated rat spleen cells, induced megakaryocyte colony formation and full maturation of megakaryocytes to cytoplasmic fragmentation. Ten percent rat serum was more effective than 20% horse serum in supporting cell growth. Greater stimulation of cell growth was provided when both serum and plasma were added to the culture medium, then when plasma was added alone. The use of a single species as the donor of bone marrow cells, spleen cells conditioned medium, plasma, and serum provided a less complex culture medium for the study of megakaryocyte development.;Rat platelet extracts (PE) and human platelet-derived growth factor (PDGF) were assayed in this culture system, in order to study the ability of these materials to modify the proliferation and/or maturation of megakaryocytic cells. The extracts prepared from 675 x 10('6) platelets or 6 ng PDGF/ml caused maximum increases over the controls of 108% or 23%, respectively, in the number of AChE+ cells. Higher concentrations of PE were found to be less effective in stimulating the maturation of AChE+ cells. It was also found that this same dose of PE caused an increase of 14.5% in the mean diameters of AChE+ cells. PDGF had no effect on the cell size. The PE also caused reduction in the number of white blood cell colonies in one series of experiments. It was not clear how and where PE or PDGF stimulated the production of new megakaryocytic cells, or inhibited the loss of these cells by cell death or by differentiation into platelets. However, the increases in the numbers and diameters of AChE+ cells induced by PE implied that substance(s) released by platelets might play a physiological role in the control of megakaryocytopoiesis.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biology
