RESTRICTION OF HUMAN ADENOVIRUS TYPE 2 REPLICATION IN CHINESE HAMSTER CELLS.
Item
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Title
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RESTRICTION OF HUMAN ADENOVIRUS TYPE 2 REPLICATION IN CHINESE HAMSTER CELLS.
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Identifier
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AAI8515652
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identifier
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8515652
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Creator
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RADNA, RACHEL LEVI.
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Contributor
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Harvey L. Ozer
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Date
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1985
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry
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Abstract
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A study of human adenovirus type 2 (Ad2) infection in Chinese hamster cells (CHO, V79) was undertaken. It was found that Ad2 infected Chinese hamster cells, however, multiplicities of infection greater than 1 pfu/cell needed. The peak of viral DNA synthesis was delayed. No progeny virus was made and the viral late genes were expressed differentially with the structural proteins synthesized at a level lower than the 100K protein. Hexon is moderately reduced and fiber is not detectible. This overall pattern is similar to that obtained in adenovirus infection of monkey cells (CV-1); however, the reduction is more pronounced in CHO. The low level of fiber in CHO could explain the failure to observe progeny virus.;The role of SV40 T antigen as a helper to adenovirus infection of Chinese hamster cells was examined. A cell line was developed in CHO which constitutively synthesized T antigen from an integrated SV40 genome. Infection of such Chinese hamster cells and conventional CHO cells with an adenovirus-SV40 hybrid (AdND1) did not result in progeny virus or increased fiber synthesis.;Chinese hamster x human cell hybrids were infected with adenovirus; some hybrids were found to correct the defect in Ad2 replication. Those hybrids which had many human chromosomes produced progeny virus. The reduced hybrids did not, however, some reduced hybrids overcame the block to fiber protein synthesis. It was not possible to assign the responsible function to a unique human chromosome.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biochemistry
