DIVERSITY OF MONOCLONAL ANTIBODIES SPECIFIC FOR SEQUENTIAL INFLUENZA VIRUS VARIANTS AND ATTEMPT TO ELICIT AN ANTI-INFLUENZA IMMUNE RESPONSE BY IMMUNIZATION WITH MONOCLONAL ANTI-IDIOTOPE ANTIBODIES AND ISOTYPE PROFILES OF ANTI-INFLUENZA ANTIBODIES IN MICE BEARING THE XID DEFECT.
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Title
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DIVERSITY OF MONOCLONAL ANTIBODIES SPECIFIC FOR SEQUENTIAL INFLUENZA VIRUS VARIANTS AND ATTEMPT TO ELICIT AN ANTI-INFLUENZA IMMUNE RESPONSE BY IMMUNIZATION WITH MONOCLONAL ANTI-IDIOTOPE ANTIBODIES AND ISOTYPE PROFILES OF ANTI-INFLUENZA ANTIBODIES IN MICE BEARING THE XID DEFECT.
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Identifier
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AAI8601687
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identifier
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8601687
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Creator
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REALE, MICHAEL ALFRED.
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Contributor
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Jerome L. Schulman
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Date
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1985
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology | Health Sciences, Immunology
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Abstract
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A panel of monoclonal antibodies specific for a corresponding panel of sequentially selected variants of influenza A/PR/8/34 virus has been established. Though the monoclonal antibodies are paratypically distinct, idiotypic relatedness has been observed. Two cross reactive idiotypes have been defined that are associated with the 7183 and S107 V(,H) gene families, respectively. The V(,K)21 group of light chains is highly represented among these antibodies. Studies of the idiotypy, and V(,H) and V(,L) genes of this panel of monoclonal antibodies suggests that both germ line encoded diversity and somatic diversifying mechanisms have contributed to their paratypic differences.;Monoclonal anti-idiotope antibodies directed to influenza specific monoclonal antibodies have been characterized in terms of their specificity. While 2 of these anti-idiotope antibodies (SN3-1A and 63-4) define idiotopes that are largely confined to PR8 virus immune sera, SN3-9A is more cross reactive in that it defines idiotopes used in both PR8 and X-31 virus immune sera. SP3-5A is an extremely cross reactive anti-idiotope reagent. Sera from mice immunized with PR8, X-31, A/Singapore(H2N2), A/Houston(H1N1), A/Chicken(H5N1) and B/Lee virus all express the SP3-5A defined idiotope. Furthermore, the SN3-9A and SP3-5A defined idiotopes were shown by adsorption studies to reside on hemagglutinin and/or neuraminidase specific antibodies.;The humoral response to PR8 virus was examined in the CBA/N and C(,3)J.xid strains of mice, both of which bear an X-linked genetic defect (xid), and in strains lacking this defect. Hemagglutination inhibiting antibody titers and measurement of virus specific antibodies by radioimmunoassay indicated that the xid defect does not impair the production of an adequate anti-influenza antibody response. However, investigation of the isotypes of PR8 virus specific antibodies disclosed a relative decrease in the levels of IgG3 and IgG1 in the xid-bearing strains. This was observed after both intraperitoneal immunization and aerosol infection. The isotype differences were not reflected in the susceptibility of these strains to influenza virus infection.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biomedical Sciences
