BOVINE BETA-CASEIN: MODEL PROTEIN TO STUDY CYSTEINE MISINCORPORATION AND PHOSPHORYLATION.

Title: BOVINE BETA-CASEIN: MODEL PROTEIN TO STUDY CYSTEINE MISINCORPORATION AND PHOSPHORYLATION.
Identifier: AAI8611340
identifier: 8611340
Creator: FLATEAU, ANNE THERESE.
Date: 1986
Language: English
Publisher: City University of New York.
Subject: Chemistry, Biochemistry | Gerontology
Abstract: Many theories have been proposed to explain aging. One theory in particular, Orgel's Error Hypothesis, has been tested here. Orgel's theory states that increasing levels of missynthesized proteins will accumulate with age and that this accumulation of protein errors is the cause of senescence and death. Bovine (beta)-casein was chosen because it did not contain the amino acid cysteine and because it had multiple phosphorylation sites. The misincorporation rate of cysteine (error rate) into "normal" (beta)-casein could be studied as well as any changes in phosphorylation with aging. Although no "error" containing (beta)-casein was isolated, an upper limit of the error rate could be estimated, based on the limits of the detection methods used. This estimate of amino acid misincorporation is too low to account for aging and thus argues against Orgel's Error Hypothesis. The degree of phosphorylation of (beta)-casein did not change with aging. However, techniques were developed which could have general applications both in aging and general research.
Type: dissertation
Source: PQT Legacy CUNY.xlsx
degree: Ph.D.
Program: Biochemistry

Media: BOVINE BETA-CASEIN: MODEL PROTEIN TO STUDY CYSTEINE MISINCORPORATION AND PHOSPHORYLATION.