AGING: A STUDY OF DNA MICROLESIONS AND METHYLATION (DEOXYURIDINE, 5-HYDROXYMETHYL).
Item
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Title
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AGING: A STUDY OF DNA MICROLESIONS AND METHYLATION (DEOXYURIDINE, 5-HYDROXYMETHYL).
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Identifier
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AAI8614686
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identifier
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8614686
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Creator
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KIRSH, MARVIN E.
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Contributor
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Aaron Lukton
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Date
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1986
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry | Gerontology
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Abstract
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Somatic mutation in terminally differentiated tissues may modulate the aging process. There is evidence that some DNA repair processes follow a path of decline as organisms age and correlations between lifespan and DNA repair capability have been established for several species. Although gross changes in DNA, such as single and double strand breaks, crosslinks, and chromosomal aberrations and reorganizations, may be of paramount importance as reflecting the decreased stability associated with increased age, mutation in the DNA base sequence may be just as important.;The work presented here involved the measurement of altered DNA bases as they may have been generated from normal bases. Altered DNA bases which may be mutagenic and which may accumulate with age were measured. In particular, deoxyuridine (dU) (a deamination product of deoxycytidine) and 5-hydroxymethyldeoxyuridine (dHMU) (an oxidation product of thymidine), were assayed in mouse tissue DNA. This assay, using an HPLC separation, is sensitive to less than 25 picomoles in DNA samples sized to 50 micromoles of deoxyribonucleosides. Neither dU nor dHMU was detected in samples of mouse tissues aged 7, 11, 19, and 31 months. In chick erythrocyte DNA, however, small amounts of dU are found, varying between DNA samples of different commercial sources. The origin of dU in these samples is unknown. dHMU was also found in chick erythrocyte DNA.;In addition to measuring dU and dHMU, 5-methyldeoxycytidine (believed to have a role in regulating gene expression) was measured as a function of age in the three tissues studied. Its levels fluctuated widely in liver and brain, but remained steady in the growing and rapidly dividing small intestinal mucosa.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biochemistry
