TRANSPLACENTAL EFFECTS OF DIETHYLSTILBESTROL ON MAMMARY GLANDS AND REPRODUCTIVE TRACTS OF FEMALE RATS.
Item
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Title
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TRANSPLACENTAL EFFECTS OF DIETHYLSTILBESTROL ON MAMMARY GLANDS AND REPRODUCTIVE TRACTS OF FEMALE RATS.
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Identifier
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AAI8801753
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identifier
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8801753
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Creator
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ROTHSCHILD, TOVA CHAYA.
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Contributor
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Elizabeth S. Boylan
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Date
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1987
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, General
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Abstract
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Female ACI rats were exposed to diethylstilbestrol (DES) in utero and followed to 10 months of age to assess effects on: (1) development of mammary glands and reproductive tract, and (2) mammary gland sensitivity to a known carcinogen (DES-cholesterol implant). Pregnant rats were injected with vehicle or DES (0.8 {dollar}\mu{dollar}g = low DES; 8.0 {dollar}\mu{dollar}g = high DES) on days 15 and 18 of gestation. Pellets containing 2.5 mg DES + 17.5 mg cholesterol (DES pellet) or 20 mg cholesterol (chol pellet) were implanted s.c. into 12-week old female offspring creating 6 experimental groups: prenatal vehicle + chol pellet (1) or + DES pellet (2); prenatal low DES + chol pellet (3) or + DES pellet (4); prenatal high DES + chol pellet (5) or + DES pellet (6).;Among rats bearing the chol pellet (group 5), 5 rats from 4 litters contained 6 palpable mammary tumors, an incidence significantly different from the zero incidence of group 1 controls. Among rats bearing the DES pellet, tumor latency decreased significantly in groups 4 and 6; tumor multiplicity at sacrifice was significantly increased in group 6 alone. These palpable mammary tumors were, almost exclusively, adenocarcinomas. Groups which received any DES (pre- or postnatally) had elongated nipples and enlarged pituitaries. Mammary glands of 74% of rats in groups 3 and 5 showed the lowest grade of differentiation, below that seen in most group 1 controls. Rats in groups 3 and 5 had an increased frequency of atypical uterine epithelia, cystically dilated uterine glands, and a thickened vaginal epithelium. Collectively, uteri in groups 2, 4 and 6 had higher incidences of luminal squamous metaplasia, cystic glands, and patches of multilayered epithelium than groups 1, 3 and 5. Vaginas from DES-treated litters exhibited a thickened vacuolated epithelium; cervices showed a convoluted morphology with numerous squamous cell nests. In comparative studies using 14-month old Sprague-Dawley rats, exposure to 120 ug DES in utero resulted in stimulation of ovarian and oviductal stroma, suppurative salpingitis, and convolutions of the cervical epithelium. Thus DES exposure in utero interferes with the normal development and differentiation of the mammary glands and reproductive organs in 2 rat strains.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
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Program
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Biology
