The effect of inhibitors and growth hormone on fatty acid oxidation in rat myocytes.
Item
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Title
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The effect of inhibitors and growth hormone on fatty acid oxidation in rat myocytes.
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Identifier
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AAI8820839
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identifier
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8820839
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Creator
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Abdel-aleem, Salah.
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Contributor
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Adviser: Horst Schulz
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Date
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1988
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry
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Abstract
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The effects of 4-bromocrotonic acid, 2-bromopalmitic acid, 3-mercaptopropionic acid, 4-pentenoic acid, and 2-tetradecylglycidic acid on the oxidation of palmitate, octanoate, and pyruvate in adult rat myocytes were studied. Since all of these compounds inhibit the oxidation of palmitate but not of pyruvate, they are specific inhibitors of fatty acid oxidation. Fifty percent inhibition of palmitate oxidation was obtained when myocytes were preincubated for 10 min with 0.1 {dollar}\mu{dollar}M 2-tetradecyl-glycidic acid, 60 {dollar}\mu{dollar}M 4-bromocrotonic acid, 60 {dollar}\mu{dollar}M 2-bromopalmitic acid, 100 {dollar}\mu{dollar}M 3-mercaptopropionic acid, or 100 {dollar}\mu{dollar}M 4-pentenoic acid. Removal of the inhibitors from the medium after preincubation relieved inhibitions caused by 3-mercaptopropionic acid or 4-pentenoic acid but did not reverse the effects of the other inhibitors. This study leads to the conclusion that 2-tetradecylglycidic acid is the compound of choice for inhibiting the mitochondrial uptake of fatty acids and thereby their oxidation while 4-bromocrotonic acid is the best irreversible inhibitor of mitochondrial {dollar}\beta{dollar}-oxidation.;The effect of growth hormone on the oxidation of oleate and docosahexaenoate was studied in rat heart mitochondria and myocytes isolated from control rats, hypophysectomized rats and hypophysectomized rats treated with growth hormone. Although growth hormone treatment increased the activity of 2,4-dienoyl-CoA reductase 2-fold, the rate at which docosahexaenoate and docosahexaenoylcarnitine are oxidized in control rats were similar to rates observed with hypophysectomized rats or hypophysectomized rats treated with growth hormone. This study suggests that the reaction catalyzed by 2,4-dienoyl-CoA reductase is not rate-limiting in the oxidation of polyunsaturated fatty acids.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
