Studies on shope fibroma virus: A tumorigenic poxvirus.
Item
-
Title
-
Studies on shope fibroma virus: A tumorigenic poxvirus.
-
Identifier
-
AAI8820885
-
identifier
-
8820885
-
Creator
-
Obom, Kristina Marie.
-
Contributor
-
Adviser: Beatriz G.-T. Pogo
-
Date
-
1988
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Biology, Microbiology
-
Abstract
-
The ability of Shope fibroma virus (SFV) to induce oncogenesis in vitro was investigated by inoculating an immortalized rabbit cell line (SIRC) with ultraviolet-irradiated virus. The resulting cell transformants displayed the characteristic properties of the malignant phenotype: lack of infectious particles, low serum requirement, high efficiency of cloning, resistance to superinfection, presence of viral DNA sequences in the nucleus, expression of viral proteins and induction of tumors in rabbits. This transformation was not stable since in all cell lines studied, a loss of the malignant phenotype was recorded close to the 50th passage.;To assess the oncogenic potential of SFV, NTH 3T3 cells were transfected with SFV DNA. The results indicated that SFV DNA could induce formation of foci in certain NIH 3T3 cell lines. Focus-derived cell lines were established to study the biological and molecular properties of the transformants. Results indicated that five of the six lines studied induced tumorigenesis in nude mice and that four lines were anchorage independent. At early passages all cell lines contained SFV DNA sequences and two of three lines tested expressed SFV DNA. It is concluded that SFV can induce the second stage of carcinogenesis and that SFV DNA can induce transformation and tumorigenic conversion of NIH 3T3 cells.;To test which region(s) of the genome is (are) necessary for transformation, NIH 3T3 cells were transfected with cloned Bam HI fragments of SFV DNA containing terminal sequences of the molecule, either alone or in combination. Only fragment C which contains the SFV growth factor could induce focus formation but transformants failed to induce tumorigenesis in nude mice, suggesting that the growth factor alone is not responsible for tumorigenic conversion of NIH 3T3 cells.;Comparative DNA studies of SFV, Indiana virus and vaccinia virus revealed the presence of mitochondria (mt) DNA in purified preparations of viral DNA. The viral and mt DNAs could be separated on the basis of their size by agarose gel electrophoresis but not by their buoyant density in isopycnic cesium chloride gradients. Southern hybridization using cloned SFV fragments as probes revealed a previously unrecognized region of homology between the SFV Bam HI fragment A and the orthopoxvirus Hind III fragments G, J and L. (Abstract shortened with permission of author.).
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
