The analysis of JB3-B, a temperature-sensitive mutant of Chinese hamster ovary cells.
Item
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Title
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The analysis of JB3-B, a temperature-sensitive mutant of Chinese hamster ovary cells.
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Identifier
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AAI8820914
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identifier
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8820914
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Creator
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Wojcik, Brian E.
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Contributor
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Adviser: Harvey L. Ozer
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Date
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1988
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, General
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Abstract
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A collection of over 200 Chinese hamster ovary cell mutants which are temperature-sensitive for growth, previously isolated by this laboratory, was analysed. Several of these mutants were identified as being primarily affected in DNA synthesis as contrasted to protein synthesis by using pulse labelling assays to determine the rates of macromolecular synthesis. The feasibility of using adenovirus and polyomavirus to facilitate their preliminary characterization has been demonstrated. One of these putative ts DNA{dollar}\sp-{dollar} mutants, JB3-B, which fails to support the replication of either of these viruses at 39.5{dollar}\sp\circ{dollar}C was further characterized through the use of adenovirus as simple model system for DNA synthesis. Evidence has been obtained which indicates that the elongation of Ad2 DNA is inhibited by the expression of the mutated function. Further data suggests that a decrease in the level of the nucleotide pools may be responsible. Additionally, genetic analysis of JB3-B indicates that it is a novel ts mutant and that the mutation is recessive to a human gene which corrects the ts phenotype. An attempt to isolate this human gene has been initiated.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
