Studies on the transformation of Escherichia coli K-12.
Item
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Title
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Studies on the transformation of Escherichia coli K-12.
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Identifier
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AAI8821101
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identifier
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8821101
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Creator
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Liveris, Dionysios.
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Contributor
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Adviser: Sharon D. Cosloy
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Date
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1988
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, General | Chemistry, Biochemistry
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Abstract
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The transformation and transfection processes are similarly affected by the same set of conditions in Escherichia coli. The chemical oxidation of the outer membrane LPS of recipient cells with periodate does not reduce either transformation or transfection, while the exposure of the recipients to proteolytic enzymes before the induction of competence, greatly inhibits transformation. The inability of vitamin B{dollar}\sb{12}{dollar} of compete with plasmid transformation suggests that the B{dollar}\sb{12}{dollar} uptake protein btu B is not a DNA uptake "channel". The association of both plasmid and phage DNAs with the polyamines spermidine and putrescine does not enhance either transformation or transfection. Transfecting phage DNAs compete very efficiently with each other and with plasmids for DNA uptake sites available on the recipient cells, while chromosomal DNA competes with transfecting DNA only by means of its mass. Recipient cells recovering from temporary protein synthesis inhibition, caused by either chloramphenicol, or tetracycline, show in the first 30 min of recovery 10-100 fold increases in both transformation and transfection efficiencies. These increases are perhaps the results of the larger number of recipient cells that are able to effectively establish the incoming DNA molecules.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
