Molecular biological study of early steps of heme biosynthesis in Escherichia coli.
Item
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Title
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Molecular biological study of early steps of heme biosynthesis in Escherichia coli.
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Identifier
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AAI9009756
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identifier
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9009756
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Creator
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Li, Jianming.
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Contributor
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Advisers: Charlotte Russell | Sharon Cosloy
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Date
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1989
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Microbiology
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Abstract
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The heme molecule is the prosthetic group of hemoglobins, cytochromes, catalases and peroxidases, and modified tetrapyrroles are the active moieties of the chlorophylls and vitamin B{dollar}\sb{12}{dollar}. The pathway of heme biosynthesis is highly conserved except for the formation of 5-aminolevulinic acid (ALA), the first common intermediate. ALA can be synthesized from glutamic acid (C{dollar}\sb5{dollar} pathway) or succinyl CoA and glycine (C{dollar}\sb4{dollar} pathway). In E. coli, the heme biosynthetic pathway consists of more than eight enzymatic steps. The genes encoding these enzymes are widely scattered on the chromosome.;An E. coli heme-requiring, hemin-permeable mutant had no detectable 5-aminolevulinic acid dehydratase (ALA D) or porphobilinogen deaminase (PBG D) activities. The gene which complemented this mutation was cloned. PBG D activity was restored to normal levels, but the activity of ALA D was 20-30 fold higher than normal. A maxicell procedure confirmed that the cloned gene was hemB. The hemB gene was sequenced. Two promoter regions, two Shine-Dalgarno sequences and two possible initiation sites were identified. Extensive homologies with yeast (36%), human liver (40%) and rat liver (40%) amino acid sequences were observed, especially in the sixteen-amino acid Zn-binding region (75%) and the four amino acids surrounding the essential lysine at the active site (100% for rate and human proteins). Analysis of promoter strength and two independent analyses of codon usage indicated that the hemB gene is moderately-expressed.;E. coli hemA gene was cloned and sequenced. Complemented mutants overproduced 5-ALA and porphyrins. The cloned sequence appears to encode a 46 kDa protein. The amino acid sequence of the cloned gene product showed no significant homologies with any cloned ALA synthase nor with any protein in two databanks. HemA gene is 41 bp away from the protein release factor 1 gene (RF1). Two ORF's oriented in the opposite direction are located upstream from hemA.;(1-{dollar}\sp{14}{dollar}C) Glutamic acid was substantially incorporated into ALA by a strain which contained hemA gene whereas (2-{dollar}\sp{14}{dollar}C) glycine was not. The synthesis of ALA was dependent on glutamic acid, ATP, NADPH, tRNA{dollar}\sp{lcub}\rm glu{rcub}{dollar} and pyridoxal phosphate. tRNA{dollar}\sp{lcub}\rm glu{rcub}{dollar} stimulated ALA synthesis in a concentration-dependent manner. Pretreatment with RNase lowered this stimulation. These results suggest that E. coli synthesized ALA by the C{dollar}\sb5{dollar} pathway.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
