Experimental and computational approaches to protein secondary structure.
Item
-
Title
-
Experimental and computational approaches to protein secondary structure.
-
Identifier
-
AAI9029972
-
identifier
-
9029972
-
Creator
-
Prestrelski, Steven Joseph.
-
Contributor
-
Adviser: Michael N. Liebman
-
Date
-
1990
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Biophysics, General
-
Abstract
-
The specific objective of this thesis was to increase the usefulness of Fourier-transform infrared spectroscopy in the determination of protein conformation in solution, though study of the conformation-sensitive amide I band. This has been accomplished through a combined computational and experimental approach. First, an algorithm has been developed for description and classification of protein secondary structures, from known protein structures, which operates independent of a predetermined structure template. Independence from this template was demonstrated to provide a more comprehensive classification of protein conformation. Fourier-transform infrared spectroscopic experiments, using derivative spectroscopy and Fourier self-deconvolution, were performed on series of bovine trypsin conformers a homologous series of proteins, the serine proteases, for which high-resolution crystal structures were available. The spectra were interpreted in the light of the enhanced description of protein conformation and these experiments have resulted in new spectra-structure correlations for the amide I infrared band allowing for a more complete interpretation of the spectral information in this region. These results allow for greater extraction of conformational information from protein infrared spectra.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
