Posttranscriptional regulation of adenovirus E1A and E1B genes during productive infection of human lymphoid cell lines.
Item
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Title
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Posttranscriptional regulation of adenovirus E1A and E1B genes during productive infection of human lymphoid cell lines.
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Identifier
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AAI9119647
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identifier
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9119647
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Creator
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Lavery, Daniel Joseph.
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Contributor
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Adviser: Selina Chen-Kiang
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Date
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1991
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Microbiology
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Abstract
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Expression of the group C adenovirus E1A and E1B genes was examined in cultured human B and T cells during productive infection. While human B and T cell lines are productively infectable by adenovirus types 2 and 5 with virus yields approaching those in HeLa cells, there was a characteristic and reproducible delay in the onset of the late infectious stage as compared with HeLa cells. To investigate this, early viral gene expression was analyzed during infection of a human B myeloma cell line, 2132. While mRNA accumulation from most viral genes was similar in 2132 and HeLa cells, E1A and E1B mRNA levels were reduced from 10- to 50-fold in 2132 cells throughout infection. Reduced E1A mRNA accumulation was reflected as well in reduced E1A polypeptide levels, as determined by immunoprecipitation. Reduced E1A and E1B mRNA accumulation was not due to reduced gene transcription, as determined by nascent RNA chain pulse labeling in isolated nuclei. Kinetic labeling of RNA to steady state levels indicated that, early after infection, nuclear events influencing the efficiency of E1A and E1B transcript utilization contributed to reduced accumulation of these mRNAs in 2132 cells. Similarly, in a human T cell line, Jurkat, E1A and E1B mRNAs accumulated in the nucleus during the late infectious stage, indicating that the regulation of E1A and E1B gene expression by nuclear RNA processing events was not restricted to 2132 cells.;In addition, the influence of adenovirus infection on the expression of the immunoglobulin {dollar}\lambda{dollar} light chain gene of 2132 cells was examined. While {dollar}\lambda{dollar} gene transcription was slightly reduced early after infection, there was a three- to five-fold increase in transcription late after infection. However, despite increased {dollar}\lambda{dollar} gene transcription, post-transcriptional events presumably mediated by E1B gene products greatly reduced {dollar}\lambda{dollar} mRNA accumulation late after infection.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
