The identification and characterization of the upstream sequences regulating the expression of the maltose fermentation genes of Saccharomyces.
Item
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Title
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The identification and characterization of the upstream sequences regulating the expression of the maltose fermentation genes of Saccharomyces.
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Identifier
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AAI9119649
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identifier
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9119649
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Creator
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Levine, Joel.
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Contributor
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Adviser: Corinne A. Michels
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Date
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1991
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Genetics | Biology, Molecular | Biology, General
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Abstract
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Maltose fermentation in Saccharomyces yeasts requires information from one of five unlinked MAL loci: MAL1, 2, 3, 4, or 6. Each locus consists of three genes encoding three proteins: maltose permease, maltase and a trans-activator protein, the MAL activator. At the MAL6 locus, the three genes are called MAL61, MAL62, and MAL63, respectively. Transcription of the MAL61 and MAL62 genes is coordinately induced by maltose and repressed by glucose; gene expression is regulated by sequences within the MAL61-MAL62 intergenic region acting in conjunction with the MAL activator.;By deletion analysis of the MAL61-MAL62 intergenic region, we show that an 81 basepair region, from basepairs -502 to -582 upstream of the MAL61 gene start codon, contains sequences necessary for the maltose induced expression of the MAL61 and MAL62 genes, the UAS{dollar}\sb{lcub}\rm MAL{rcub}{dollar}. This sequence contains two copies of an 11 basepair dyad which may be the active elements of the UAS{dollar}\sb{lcub}\rm MAL{rcub}{dollar}. Using heterologous gene plasmid constructs, we demonstrate that the sequences of the UAS{dollar}\sb{lcub}\rm MAL{rcub}{dollar} are sufficient for maltose inducibility of the MAL62 gene and that this regulated expression requires a functional MAL activator.;Our results suggest that the MAL61-MAL62 intergenic region contains additional distinct elements which function to precisely regulate MAL61 and/or MAL62 gene expression. Among these are repressing sequences including a glucose-responsive element, located between basepairs -583 and -638, which is partially responsible for mediating glucose repression of MAL62 gene expression.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
