Photon correlation spectroscopy of synthetic phospholipid vesicles during osmotic swelling.
Item
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Title
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Photon correlation spectroscopy of synthetic phospholipid vesicles during osmotic swelling.
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Identifier
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AAI9130369
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identifier
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9130369
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Creator
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Rutkowski, Christopher Anthony.
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Contributor
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Adviser: Thomas H. Haines
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Date
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1991
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biophysics, General | Chemistry, Biochemistry | Engineering, Materials Science
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Abstract
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Osmotic swelling experiments were conducted on a variety of preparations of "uniform", unilamellar vesicle systems. The synthetic lipid preparations included both vesicles produced by extrusion through polycarbonate ultrafiltration membranes and vesicles produced by the pH adjustment method. The vesicles were monitored by photon correlation spectroscopy during swelling as the osmolarity of the external solution was decreased. Large unilamellar vesicles produced from acidic lipids using the pH adjustment technique were highly polydisperse and did not swell in a manner that permitted the computation of an elastic modulus, presumably due to the polydispersity of the preparations. Analysis of osmotic swelling of extruded unilamellar vesicles has allowed us to assign elastic moduli for bilayers of dioleoylphosphatidyl choline and dioleoylphosphatidyl glycerol, in the range 5 {dollar}\times{dollar} 10{dollar}\sp8{dollar} to 12 {dollar}\times{dollar} 10{dollar}\sp8{dollar}, and 3 {dollar}\times{dollar} 10{dollar}\sp8{dollar} to 6 {dollar}\times{dollar} 10{dollar}\sp8{dollar} dyn/cm{dollar}\sp2{dollar}, respectively. Membranes derived from bovine submitochondrial particles and rye plasma membrane did not produce evidence of swelling when subjected to similar protocols.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
