Serine threonine kinases as signaling intermediates inv-Src-induced gene expression.

Title: Serine threonine kinases as signaling intermediates inv-Src-induced gene expression.
Identifier: AAI9207114
identifier: 9207114
Creator: Qureshi, Sajjad Aslam.
Contributor: Adviser: David A. Foster
Date: 1991
Language: English
Publisher: City University of New York.
Subject: Biology, Cell | Biology, Molecular | Biology, Genetics
Abstract: v-Src induces expression of two primary response genes, TIS-10 and Egr-1. Depleting cells of protein kinase C (PKC) blocked v-Src-induced TIS10 expression, but had no effect on v-Src-induced Egr-1 gene expression. In addition, the induction of TIS10 and Egr-1 by v-Src could be distinguished pharmacologically. Therefore, the v-Src induces gene expression via at least two distinguishable pathways; one that is dependent upon PKC and one that is independent of PKC. A kinase-deficient c-Raf-1 that function as a dominant-negative mutant of c-Raf-1 inhibited the activation of Egr-1 gene by v-Src. This inhibition was overcome by overexpression of wild type c-Raf-1. Consistent with the involvement of c-Raf-1 in Egr-1 promoter activation, an activated Raf kinase also activated the Egr-1 promoter. Thus, v-Src-induced signaling involves the activation of at least two serine/threonine kinases, PKC and c-Raf-1 that leads to the induction of two different primary response genes.
Type: dissertation
Source: PQT Legacy CUNY.xlsx
degree: Ph.D.

Media: Serine threonine kinases as signaling intermediates inv-Src-induced gene expression.