The effect of transplacental exposure to diethylstilbestrol on mammary gland development in the peripubertal female ACI rat.
Item
-
Title
-
The effect of transplacental exposure to diethylstilbestrol on mammary gland development in the peripubertal female ACI rat.
-
Identifier
-
AAI9207132
-
identifier
-
9207132
-
Creator
-
Vassilacopoulou, Dido.
-
Contributor
-
Adviser: Elizabeth S. Boylan
-
Date
-
1991
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Biology, Cell | Biology, Animal Physiology
-
Abstract
-
Female ACI rats were exposed to diethylstilbestrol (DES) in utero, to evaluate effects on: (1) mammary gland development; (2) mammary gland sensitivity to natural and synthetic estrogens, and (3) mammary gland sensitivity to endogenous epidermal growth factor (EGF). Additionally, a study was undertaken to examine certain aspects of morphological and physiological differences observed between rats displaying intact or unilateral agenesis of the genital tract. Pregnant rats were injected with vehicle (sesame oil) or DES (total dose, 8.0 {dollar}\mu{dollar}g) on days 15 and 18 of gestation. DES-exposed and control offspring were ovariectomized at 34 days of age and sacrificed at day 53 to ascertain the morphology of the mammary glands in peripubertal rats. A separate group of DES-exposed and control animals was implanted with Elvax pellets containing estradiol 17B (E2) or DES at 5 or 11 ng adjacent to the third mammary gland pair. Furthermore, an additional group of rats was subjected to bilateral sialoadenectomy at the day of ovariectomy to remove the major source of endogenous EGF. Mammary glands of DES-exposed animals exhibited atypical mammary gland morphology, tending to hypo- or hyper-differentiation, and seemed to be refractory to stimulation by DES or E2, when compared to controls. Sialoadenectomy had no apparent effect on mammary gland morphology in either the DES-exposed or vehicle-exposed groups.;Uterine and mammary morphological differences were observed between rats with intact genital tracts and unilateral agenesis of the reproductive tract. The timing of vaginal opening was found to be accelerated in rats with unilateral agenesis. These pronounced differences resulted in exclusion of all rats with unilateral agenesis from the tabulation of data on the effects of prenatal exposure to DES which were described above.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
