Molecular analyses of a transfected partial humanrDNA repeat unit in human fibroblasts: NOR formation.
Item
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Title
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Molecular analyses of a transfected partial humanrDNA repeat unit in human fibroblasts: NOR formation.
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Identifier
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AAI9218236
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identifier
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9218236
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Creator
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Hadjiargyrou, Michael.
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Contributor
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Adviser: Ann S. Henderson
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Date
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1992
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Genetics
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Abstract
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A functional comparison was used to investigate the relationship between ribosomal DNA (rDNA) and the function and formation of the nucleolus, as well as corollary events, such as satellite associations. Cloned human rDNAs were isolated from a cosmid library and characterized. Analyses showed that one clone contained an intact transcribed spacer region and most of the nontranscribed spacer region. The second contained a transcribed spacer and much of the nontranscribed region, but lacked the promotor. These were transfected into human fibroblasts. Molecular analyses determined that one to two copies of the transfected DNA is present in the genomic DNA of the transfected cells. Preliminary results with silver staining showed that the rDNA containing a promotor region is transcribed. Further molecular and cytological analyses demonstrated that this rDNA displays Nucleolar Organizer Region (NOR) activity and participates in satellite associations. In contrast transfected rDNA lacking a promotor is not transcribed, and does not participate in NOR activity. We conclude from this study that tandem repetition is not an absolute requirement for rDNA function and that transfected rDNA containing a promotor region can establish an active NOR region.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
