Cellular morphogenesis and the cytoskeleton in amphibian erythrocytes.
Item
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Title
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Cellular morphogenesis and the cytoskeleton in amphibian erythrocytes.
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Identifier
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AAI9224815
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identifier
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9224815
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Creator
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Ginsburg, Mary Fuday.
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Contributor
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Adviser: William D. Cohen
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Date
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1992
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Cell
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Abstract
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"Two things are required from every specialized treatise: it should clarify its subject and, in the second place, but actually more important, it should tell us how and by what methods we can attain it and make it ours." (Pseudo-Longinus, prob 1st century, On the Sublime).;The nucleated erythrocytes of non-mammalian vertebrates provide an excellent model system for studying cytoskeletal function during cellular morphogenesis. Previous work by several laboratories has suggested that the marginal band is important in establishing cell shape as such cells undergo transition from mitiotic erythroblast to flattened ellipse; however, much of this work has been done by manipulation of mature cells of various animals. The research presented herein attempted to extend such findings by an analysis of marginal band development and its possible correlation with cell morphogenesis during maturation of larval axolotl erythrocytes, with emphasis on the population in the spleen, which is not connected to the general circulation at early developmental stages in this organism. Approaches included tabulation of cell shapes as a function of larval age and a variety of morphological studies of erythrocytic cells at different stages of development, using such techniques as scanning electron microscopy of whole cells, transmission electron microscopy and immunofluorescence of cytoskeletons, and cell culture.;The results were consistent with the hypothesis that the marginal band plays a role in determination of cell shape during development and were used as the basis for a preliminary model for the morphogenesis of these erythrocytes. More importantly, however, the work extended the excellent work of Duprat and Flavin (1976) in demonstrating the value of the axolotl spleen as a developmental system deserving of study. It is hoped that this dissertation may serve as a function for further inquiry, even as did theirs.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
