A partial human ribosomal DNA repeat unit transfected into a human fibroblast cell line functions as a nucleolar organizing region.
Item
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Title
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A partial human ribosomal DNA repeat unit transfected into a human fibroblast cell line functions as a nucleolar organizing region.
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Identifier
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AAI9224836
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identifier
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9224836
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Creator
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McDowell, Kathi Anne.
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Contributor
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Adviser: Ann S. Henderson
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Date
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1992
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Genetics
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Abstract
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The role of rDNA in nucleolar formation was investigated by creating a "new" rDNA site within the human genome. Human rDNA, isolated from a cosmid library, was transfected into human fibroblast cells. One transfectant (ML1 DNA) contained the rDNA transcription unit and most of the flanking non-transcribed spacer DNA. A second (ML4 DNA) contained the transcription unit and most of the non-transcribed spacer, but lacked a promoter region. One copy of ML1 DNA inserted into the pericentromeric region of the p-arm of human chromosome 3; ML4 DNA was localized to the q-arm of chromosome 4. The insertion site containing the promotor was transcriptively active, but there was no detectable transcription at the site lacking a promotor. The insertion site containing the rDNA promotor was present in nucleoli and participated in resultant satellite associations. This study shows that the rDNA promotor region is required in nucleolar formation and that tandem repetition of rDNA is not an absolute requirement for participation in nucleolar activity.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
