The study of pathological changes in the islets of Langerhans in hamsters infected with the 139H strain of scrapie.
Item
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Title
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The study of pathological changes in the islets of Langerhans in hamsters infected with the 139H strain of scrapie.
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Identifier
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AAI9224867
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identifier
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9224867
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Creator
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Ye, Xuemin.
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Contributor
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Adviser: Richard I. Carp
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Date
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1992
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Language
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English
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Publisher
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City University of New York.
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Subject
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Health Sciences, Pathology | Biology, Animal Physiology | Biology, Neuroscience
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Abstract
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The purpose of this study was to examine pathological changes in the islets of Langerhans in hamsters infected with the 139H strain of scrapie. In comparing 139H-infected and control hamsters, the following was found: (1) The number and total area of islet profiles was greater in 139H-infected than in control animals. (2) The ratio of B:A:D:F cells in islets was changed from 27:5:1:0.04 in controls to 122:7:1:0.04 in 139H-infected hamsters. The increase in B cells would account for the islet enlargement and the hypoglycemia-hyperinsulinemia previously observed in 139H-infected hamsters. (3) The optical density of immunostained insulin containing cells was decreased, suggesting that the concentration of insulin in B granules or the number of B granules was decreased, or both. (4) There were extensive histopathological changes in the islets and pituitary in 139H-infected hamsters. These changes included cellular hypertrophy, cellular atrophy, vacuolation, nuclear swelling, pyknosis, karyorrhexis and karyolysis. Some of these changes were also seen in hamsters infected with the 263K strain of scrapie, but at a much reduced incidence. (5) Abnormal structures which I termed blood vessel cores (BVCs) were seen in the islets of 139H-treated hamsters. I developed new terms to described pathological phenomena seen in 139H-infected hamsters, including the abnormal PAS-positive substance (PPS), the linkage-reaction, linkage-inflammatory cells, localized vacuolization (LV), diffuse vacuolization (DV), and pathological pattern synchronism. I also proposed the accordion effect concept to explain a function of the extracellular spaces in the islets. (6) I observed that the pattern of positive cells immunostained with antibodies against several hormones was changed in the vacuolated areas of the pituitaries of 139H-infected hamsters. (7) There was a significant increase in the number of corticotropin-releasing factor (CRF) immunostained neurons in hypothalami of 139H-infected compared to control hamsters. (8) There was a significant decrease in vasopressin (VP) neurons in the lateral hypothalami (LHy), but not in the dorsalmedial hypothalami (DMH) and supraoptic nuclei (SON), in 139H-infected compared to control hamsters. (9) I used three-dimensional modeling to demonstrate the changes of CRF- and VP-immunostained neurons in the 139H-infected hamsters.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
