Quantitative neurochemical analysis of the macaque hypothalamic paraventricular nucleus.
Item
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Title
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Quantitative neurochemical analysis of the macaque hypothalamic paraventricular nucleus.
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Identifier
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AAI9315461
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identifier
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9315461
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Creator
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Ginsberg, Stephen David.
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Contributor
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Adviser: John H. Morrison
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Date
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1993
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Neuroscience | Biology, Anatomy
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Abstract
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In an attempt to correlate morphologic organization with defined physiologic responses, a series of six specific aims were performed to assess the chemoarchitecture of the macaque hypothalamic paraventricular nucleus. Specific aim 1 examined the regional distribution of noradrenergic-containing structures throughout the rostrocaudal extent of the monkey hypothalamus using dopamine-{dollar}\beta{dollar}-hydroxylase immunohistochemistry. In specific aim 2, quantification of dopamine-{dollar}\beta{dollar}-hydroxylase-immunoreactive varicosities was performed within both magnocellular and parvicellular regions of the monkey hypothalamic paraventricular nucleus. Quantification of noradrenergic varicosities in apposition to immunohistochemically-identified magnocellular neurons within the hypothalamic paraventricular nucleus was performed in specific aim 3 using confocal laser scanning microscopy combined with double label immunofluorescence. Specific aims 4-6 assessed the potential affects of the early environmental insult of social deprivation on three neurotransmitter systems within the monkey hypothalamus. In specific aim 4, quantitative analysis of dopamine-{dollar}\beta{dollar}-hydroxylase-immunoreactive varicosities was performed within the hypothalamic paraventricular nucleus in both socially reared and socially deprived rhesus monkeys. In specific aim 5, a quantitative assessment of the tuberoinfundibular dopaminergic neurons within the hypothalamic paraventricular nucleus and arcuate nucleus using tyrosine hydroxylase immunohistochemistry was performed in socially reared and socially deprived monkeys. Additionally, an assessment of the potential affects of rearing condition on corticotropin-releasing factor-immunoreactive neurons was performed in specific aim 6. These experiments give insight into the noradrenergic input to the hypothalamus with subsequent quantitative analyses within a restricted region, as well as assessing the potential affects of an experimental manipulation on neurotransmitter-specified systems. Taken together, the aims of this project also yielded an assessment of the overall morphologic organization of the hypothalamic paraventricular nucleus in relation to several physiologically functional circuits.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
