Studies on the effect of 8-Cl-cAMP on the proliferation of mammalian cells.

Title: Studies on the effect of 8-Cl-cAMP on the proliferation of mammalian cells.
Identifier: AAI9315463
identifier: 9315463
Creator: Han, Zhiyong.
Contributor: Adviser: James H. Wyche
Date: 1993
Language: English
Publisher: City University of New York.
Subject: Biology, Cell
Abstract: The cyclic AMP analog, 8-Cl-cAMP, was originally thought to inhibit the proliferation of various tumor cell lines via its selective interaction with type II cAMP-dependent protein kinase (type II PKA). In addition, it was concluded that the inhibitory effect of 8-Cl-cAMP on cell proliferation was selective for tumor-derived or transformed cells. In this study, we have confirmed other's observation that 8-Cl-cAMP can effectively inhibit the proliferation of various types of cultured cells. However, our study also indicates that (1) 8-Cl-cAMP inhibits the proliferation of tumor-derived, transformed, and non-transformed cells; (2) the inhibitory effect of 8-Cl-cAMP on cell proliferation is dependent on the presence of cyclic nucleotide phosphadiesterase and nucleotide 5{dollar}\sp\prime{dollar}-phosphatase; and (3) 8-Cl-cAMP is hydrolyzed and converted into 8-Cl-adenosine, and it is 8-Cl-adenosine but not 8-Cl-cAMP that exerts the inhibitory effect on the proliferation of various cells. Therefore, the inhibition of cell proliferation by 8-Cl-cAMP is not mediated through type II PKA.
Type: dissertation
Source: PQT Legacy CUNY.xlsx
degree: Ph.D.

Media: Studies on the effect of 8-Cl-cAMP on the proliferation of mammalian cells.