Failure of secretion of a human B cell differentiation factor by patients with common variable immunodeficiency.
Item
-
Title
-
Failure of secretion of a human B cell differentiation factor by patients with common variable immunodeficiency.
-
Identifier
-
AAI9405539
-
identifier
-
9405539
-
Creator
-
Kazbay, Kasim.
-
Contributor
-
Adviser: Lloyd Mayer
-
Date
-
1993
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Health Sciences, Immunology | Biology, Cell
-
Abstract
-
Common variable immunodeficiency is a heterogeneous disorder characterized by absent, low, or ineffective antibody secretion in vivo. To date, a number of in vitro defects have been described, including diminished circulating B cells, intrinsic B cell defects, absence of T cell help, and excessive T cell suppression. In this study B cells from a majority of patients (84%) responded in vitro to isolated B cell differentiation factors (BCDF) with normal or near normal Ig secretion. These data suggest that the defect in these patients may reside in the T cell rather than the B cell. We activated CVI and normal control T cells with various stimuli including PHA, anti-CD3 or anti-CD2 mAbs and measured T cell proliferation and cytokine secretion. Despite normal proliferative responses to all stimuli, BCDF secretion was absent in 72% of patients. Interestingly, all patients who failed to secrete BCDF, were capable of responding to BCDF in vitro by normal or even enhanced Ig secretion. BCDF deficiency existed alone or in concert with defects in IL2 and/or IL6 secretion. These findings suggest that defects in T cell activation resulting in abnormal secretion of potent BCDFs may underlie the immunodeficiency in CVI.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
