The phosphonic acid analog of phosphatidylglycerol phosphate: Influence on Escherichia coli growth and physiology.
Item
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Title
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The phosphonic acid analog of phosphatidylglycerol phosphate: Influence on Escherichia coli growth and physiology.
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Identifier
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AAI9405540
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identifier
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9405540
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Creator
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Ke, Lei.
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Contributor
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Adviser: Burton Tropp
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Date
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1993
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry | Biology, Microbiology | Biology, Animal Physiology
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Abstract
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The growth of Escherichia coli is only slightly inhibited when the cells are incubated with 20 {dollar}\mu{dollar}M 3,4-dihydroxybutyl-1-phosphonate (DBP){dollar}\sp1.{dollar} However, the cells lose viability when exposed to a combination of 20 {dollar}\mu{dollar}M rac-DBP and 20 mM magnesium or calcium ions. Magnesium ions stimulate the incorporation of DBP into (1,2-diacyl)-sn-glycerol- scD-4{dollar}\sp\prime{dollar}-phosphoryloxy-{dollar} 3\sp\prime{dollar}-hydroxybutyl-1{dollar}\sp\prime{dollar}-phosphonate, the phosphonate analog of phosphatidylglycerol phosphate.;Much higher DBP concentrations are needed to block the growth of a pgsA3 mutant than to block the growth of an isogenic wild-type strain. The DBP-treated pgsA mutant also has a much higher survival rate when stored in the cold than does the DBP-treated wild-type strain. Furthermore, the pgsA3 mutant grows normally in the presence of DBP and magnesium chloride. Treatment with DBP and magnesium ions does not appear to disrupt the cell's inner or outer membranes. The combination of DBP and magnesium chloride does not have serious metabolic consequences for the pgsA3 mutant strain but inhibits macromolecular, phosphoglyceride and ATP synthesis in wild-type strains. A combination of 20 {dollar}\mu{dollar}M rac-DBP and 0.5 mM spermidine or 0.125 mM spermine is bacteriostatic. These studies suggest that the PGP analog contributes to DBP's bacteriostatic effect when the growth medium contains low concentrations of magnesium or calcium ions and is responsible for its bactericidal effect when the medium contains high concentrations of these ions.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
