A study of the effects of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on mesencephalic dopaminergic neurons in culture: Glia-mediated protection against neurotoxins and stimulation of regrowth.
Item
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Title
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A study of the effects of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on mesencephalic dopaminergic neurons in culture: Glia-mediated protection against neurotoxins and stimulation of regrowth.
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Identifier
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AAI9405571
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identifier
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9405571
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Creator
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Park, Tina Hairyung.
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Contributor
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Adviser: Catherine Mytilineou
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Date
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1993
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Neuroscience
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Abstract
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Many peptide trophic factors have been shown to be able to maintain the survival and promote the recovery of injured central nervous system neurons. We investigated the effects of two trophic factors, epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), on rat embryonic mesencephalic cultures in order to test whether or not they could (a) modify the damage caused by the specific dopaminergic neurontoxins 1-methyl-4-phenylpyridinium (MPP{dollar}\sp+){dollar} and 6-hydroxydopanine (6-OHDA), or (b) affect the ability of the dopaminergic neurons to recover following toxin-induced damage.;Our results demonstrate that both EGF and bFGF protect dopaminergic neurons against the neurotoxic effects of MPP{dollar}\sp+{dollar} and 6-OHDA, and also promote regenerative processes of injured dopaminergic neurons. We provide evidence that these effects are mediated by astrocytic glial cells. Therefore, our studies suggest that astrocytic glial cells synthesize and secrete factors which enhance the biochemical and morphological development of dopaminergic neurons in vitro and increase the resistance of these cells to specific neurotoxins.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
