Mechanism of decidualization of human endometrial stromal cells in vitro.
Item
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Title
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Mechanism of decidualization of human endometrial stromal cells in vitro.
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Identifier
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AAI9405590
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identifier
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9405590
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Creator
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Tang, Baiqing.
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Contributor
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Adviser: Erlio Gurpide
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Date
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1993
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Cell | Chemistry, Biochemistry | Biology, Animal Physiology
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Abstract
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Decidualization of the human endometrial stroma plays an important supporting role in embryo implantation and subsequent pregnancy. This process involves changes in cell morphology and the expression of products characteristic of decidual tissue. In order to investigate the molecular mechanism and regulation of the differentiation events, human endometrial stromal cells were exposed to different hormones and agents and decidualization of the cells was evaluated by observation of their morphologic change to decidual phenotype and determination of the expression of decidual products, such as prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1), heat shock protein-27 (HSP-27), desmin, laminin, and fibronectin.;Induction of PRL was detected in the cells treated with dibutyryl cAMP (db-cAMP) by using immunocytochemistry, immunoprecipitation and Western blotting procedures. Time and concentration dependence of db-cAMP induced increases PRL protein output was determined by using RIA and ELISA. Dibutyryl-cAMP also promoted a time dependent increase in levels of PRL mRNA, normalized to cyclophilin mRNA. Prolactin production was also observed in stromal cells cultured with 8-Br-cAMP or forskolin. MPA (medroxyprogesterone acetate) synergistically enhanced the inductive effects of db-cAMP, 8-Br-cAMP or forskolin on PRL production, as measured by Western blotting or ELISA. Transcription studies indicated that MPA also increased the rate of the PRL gene transcription in stromal cells cultured with db-cAMP. Estradiol (E{dollar}\sb2{dollar}) had no significant effect on db-cAMP induction of PRL production. In addition to its effects of PRL synthesis, db-cAMP induced morphologic change and expression of IGFBP-1, HSP-27, desmin, laminin, and fibronectin in cultured stromal cells. Intracellular cAMP levels was elevated in the stromal cells cultured with gonadotropins. Furthermore, the treatment with gonadotropins induced PRL expression and morphologic changes of the stromal cells.;These results suggest a model to dissect the molecular mechanisms of decidualization involving gonadotropins, cAMP, steroids, and PRL expression. The synergistic regulation of PRL production in human endometrial stromal cells by MPA and cAMP suggests that these compounds regulate PRL expression and decidualization through interdependent pathways. Our results indicate that a cAMP mediated signal transduction pathway is involved in decidualization of human endometrial stromal cells.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
