Purification and characterization of brain-specific small cytoplasmic BC1 and BC200 RNPs.
Item
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Title
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Purification and characterization of brain-specific small cytoplasmic BC1 and BC200 RNPs.
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Identifier
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AAI9417449
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identifier
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9417449
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Creator
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Cheng, Jr-Gang.
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Contributor
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Adviser: Jurgen Brosius
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Date
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1994
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Neuroscience
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Abstract
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The brain-specific small cytoplasmic BC1 and BC200 RNA contain many interesting features. They are first known RNA polymerase III transcripts with neuronal specificity. The expression of BC RNA is regionally and developmentally regulated. Furthermore, it is one of few RNAs with is actively transported into dendrites. Two type of BC small RNAs have been identified which is conserved in different animal order, i.e., BC1 RNA in the rodents and BC200 RNA in the primates. The role of BC RNAs is unknown, however, they may be involved in translational regulation and RNA sorting. Characterization and purification of those scRNPs is used to approach their physiological function.;Several piece evidence support the notion that BC1 and BC200 RNA exists as an RNP in cytoplasm of neuron. First, the BC RNAs in the brain extract have lighter buoyant density than theirs naked RNA have. Second, the BC RNAs in the brain extract (BC1 RNP{dollar}\sim{dollar}8.6 S; BC200 RNP{dollar}\sim{dollar}11.2 S) have different S value from their naked RNA (BC1 RNA{dollar}\sim{dollar}6 S; BC200 RNA{dollar}\sim{dollar}7.6 S). Further studies suggest that other observation such as the behavior of BC RNP in the electrophoresis field and the proteinase K and RNase A treatment also support the same idea.;Much progress have been made toward the goal of purification of BC1 RNP. It has been studied the behavior of BC1 RNP with different fractionation methods and integrated BC1 RNP purification scheme. Although it has been reached 2500 fold enrichment starting from brain crude extract, there are high risk for BC1 RNP falling apart and contamination. It have been solved most of difficulties during BC1 RNP purification. In addition to conventional biochemical approach, several new strategies and new methods also have been applied to facilitate the identification and purification of BC1 RNA assorted proteins.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
