The effects of early maternal deprivation on adult behavior in Sprague Dawley rats.
Item
-
Title
-
The effects of early maternal deprivation on adult behavior in Sprague Dawley rats.
-
Identifier
-
AAI3115276
-
identifier
-
3115276
-
Creator
-
Morgan, Catherine J.
-
Contributor
-
Adviser: Gerald Turkewitz
-
Date
-
2004
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Psychology, Developmental | Psychology, Psychobiology | Psychology, Experimental
-
Abstract
-
Early Maternal Deprivation reliably derails the developmental trajectory of adult stress responses. Without early environmental intervention Hypothalamic-Pituitary Axis function is permanently altered. This work investigates maternal deprivation procedural mechanisms and seeks to uncover some underlying relationships between procedure and resulting shifts in adult behavioral outcome. Male and female rats were exposed to one of two different Isolation Treatment (IT) procedures both utilizing a 1-hour for 8-days (PND 2 thru PND 9) deprivation from the dam and siblings, either with or without a standard auditory white noise mask. Before/after treatment pup weights were measured; adult weights at two ages were related to perinatal weights. Also, influence of psychostimulant drug was examined.;Utilizing a 2-Isolation x 2-Drug x 2-Sex Design, two adult 30-minute tests were videotaped: (1) Open Field Box; and (2) Post baseline. Either a "challenge" 2.0 mg/kg dose of Amphetamine or .09% physiological Saline was administered 15-minutes prior to placement in the test apparatus; anxiety behavior was scored.;All IT subjects receiving Amphetamine Challenge exhibited significantly higher stress behavior scores, across all measures, compared with No-Isolation Animal Facility Reared Controls. Direction and strength of response to Amphetamine pre-stressor depended on type of IT received: No-Auditory IT subjects had exaggerated, higher drug responses compared with all other subjects regardless of condition, while Amphetamine Auditory IT subjects had activity levels (1) lower than Amphetamine Controls, (2) lower than No-Auditory IT Amphetamine Conditions. Only No-Auditory IT subjects exhibited significantly different scores across all measures when receiving only Saline pre-test.;Significant differences existed in pup, and in adult weights, between: (1) Treatment and Controls, (2) Drug Conditions, (3) types of IT, (4) before/after deprivation. All IT pups gained less weight than Controls during 8-day treatment, and before-after treatment on PND 5; unexpectedly, pups experiencing No-Auditory IT compared to Auditory IT's, gained less weight PND 2-PND 9, and before-after treatment on PND 5. This relationship in Treatment v. Control weight differences, and between types of IT, persisted into adulthood.;Behavioral measures revealed a differential impact on adult stress-responses, on pup-adult weight, dependant on Isolation Treatment procedures.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
