Regulation of gonadotropin releasing hormone gene expression in a mouse hypothalamic neuronal GT1 cell line.
Item
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Title
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Regulation of gonadotropin releasing hormone gene expression in a mouse hypothalamic neuronal GT1 cell line.
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Identifier
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AAI9417517
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identifier
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9417517
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Creator
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Yeo, Tracy Teck Si.
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Contributor
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Adviser: James L. Roberts
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Date
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1994
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Neuroscience | Biology, Molecular | Health Sciences, Obstetrics and Gynecology
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Abstract
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Gonadotropin releasing hormone (GnRH) is the primary neuropeptide regulating vertebrate reproductive functions. Numerous factors are known to regulate GnRH release through direct or indirect neuronal synaptic inputs or by surrounding non-neuronal cells. However, the low abundance and sparse distribution of GnRH neurons have limited the understanding of the regulation of GnRH biosynthesis by these factors. The complexity of neuronal inputs and cellular compositions surrounding the GnRH neurons have also made it difficult to determine whether the effects of these factors on the GnRH system are through direct action on the GnRH neurons or are secondary, through interneurons or surrounding non-neuronal cells. During my graduate study with Dr. James L. Roberts, we have taken the advantage of the recent production of a mouse hypothalamic neuronal GnRH-secreting cell line, the GT1 cells, to explore the cellular and molecular mechanisms by which the GnRH neuronal functions are regulated.;What I present here in this dissertation thesis contains the bulk of experimental data generated in my graduate study on establishing the GT1 system and assay methods for the elucidation of GnRH gene expression in response to normonal regulation. I have characterized the basic features of the GnRH gene transcripts and defined assay methods to elucidate the hormonal regulation of GnRH gene expression along its mRNA biosynthetic pathway which can act in concert to regulate the final levels of mRNA templates to be translated into GnRH prohormone. I have then studied the effects of the activation of two major intracellular second messenger pathways, cAMP/protein kinase A (PKA) and diacylglycerol/protein kinase C (PKC), by which the effects of numerous neurotransmitters and hormones are mediated, on GnRH gene expression. The establishment of this system will provide useful information on advancing our understanding on the neuroendocrine control of the GnRH system.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
