Effects of extremely low frequency electromagnetic fields on transcription and translation of a transformed human fibroblast cell line.
Item
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Title
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Effects of extremely low frequency electromagnetic fields on transcription and translation of a transformed human fibroblast cell line.
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Identifier
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AAI9521274
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identifier
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9521274
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Creator
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Gold, Steven.
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Contributor
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Adviser: Ann S. Henderson
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular
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Abstract
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The purpose of this study was to examine the transcriptional and translational responses of an SV40-transformed human fibroblast cell line, HAL, exposed to a 20-minute, 60Hz electromagnetic field. SV40 mRNA levels were found to increase in both Helmholtz-aiding and solenoid exposure systems based on measurements of the viral large-T antigen. The translational product of large-T antigen was seen to increase in a Helmholtz-aiding system.;Large-T antigen is known to increase heat shock 70 (hsp70) transcription. Using a reverse transcription-polymerase chain reaction (RT-PCR) protocol, a comparison of the heat shock and electromagnetic responses in the HAL cell line was undertaken based on earlier work which suggested a correlation between electromagnetic exposure and heat shock in cells. Transcript levels of hsp70, c-fos, c-myc, p53 and c-jun were measured in a 20-minute, 60Hz field. HAL cells were heat-shocked for periods of 4 hrs and 20 minutes, respectively, with subsequent measurements made of hsp70, c-fos, c-myc and c-jun transcript levels. EM-exposed cells elicited a notable increase in c-myc and p53 mRNA levels and essentially no change in hsp70 levels; heat-shocked cells resulted in large increases of hsp70 following either 4hr or 20-minute incubation. In summary, the transcriptional patterns observed in cells exposed to electromagnetic fields and those that were heat shocked differed, although evidence is presented that electromagnetic exposure represents a form of cellular stress.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
