Functional analysis of the roles of the N:P protein complex and the C proteins of vesicular stomatitis virus in the replication of the viral genome.
Item
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Title
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Functional analysis of the roles of the N:P protein complex and the C proteins of vesicular stomatitis virus in the replication of the viral genome.
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Identifier
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AAI9521292
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identifier
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9521292
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Creator
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Lock, Martin William.
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Contributor
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Adviser: Richard Peluso
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology | Biology, Molecular
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Abstract
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Molecular events which distinguish the replication of the negative sense RNA genome of vesicular stomatitis virus (VSV) from viral transcription have been addressed. In contrast to transcription which entails the discontinuous synthesis of monocistronic mRNA, replication involves the synthesis of a full length copy of the genome and concurrent encapsidation of the nascent RNA by the nucleocapsid protein. In vitro studies performed here indicate that the recognized encapsidation substrate, the nucleocapsid protein:phosphoprotein (N:P) complex, is not able to directly and independently encapsidate nascent genomic RNA sequences. Rather, results from an in vivo replication system suggest that the N:P protein complex interacts with the viral RNA polymerase during replicative RNA synthesis and that this interaction may facilitate the delivery of the N protein to the nascent genome. A direct enzymatic role of the P protein component of the encapsidation complex in the replicative RNA synthetic reaction is not supported by the data. The effect of the recently demonstrated C proteins of VSV on viral RNA synthesis has been assessed using in vitro transcription and replication systems. In these systems, the C proteins are shown to exert negative and positive regulation of transcription and replication respectively. These results imply that the C proteins are important factors in the switch from the transcriptive to the replicative mode of VSV RNA synthesis.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
