Gonadotropin-releasing hormone receptor: Cloning, characterization, and mutational investigation.
Item
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Title
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Gonadotropin-releasing hormone receptor: Cloning, characterization, and mutational investigation.
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Identifier
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AAI9521326
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identifier
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9521326
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Creator
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Zhou, Wei.
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Contributor
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Adviser: Stuart C. Sealfon
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Neuroscience | Health Sciences, Pharmacology | Biology, Molecular | Biology, Animal Physiology
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Abstract
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Gonadotropin-releasing hormone (GnRH) plays a critical role in regulating the function of the mammalian reproduction system. GnRH exerts its effects by interacting with the gonadotropin-releasing hormone receptor (GnRHR). In this thesis work, molecular techniques were used to investigate the structure and function relationship of the GnRHR. The body of work consists of two major components: one encompassing the molecular cloning of the mouse GnRHR and the other, the characterization of a series of mutant receptors created by site-directed mutagenesis. The first part covers both the cDNA and the genomic cloning of the mouse GnRHR as well as the identification of variant transcripts of the receptor gene found in a mouse pituitary tumor cell line. The second part of the thesis involves the analysis of a series of residues important for receptor function. Several residues have been characterized that have critical roles in receptor binding and activation and the results support an interaction between transmembrane helices 2 and 7. These studies provide experimental evidence for defining the molecular model of the GnRHR and should lead to further understanding of the mechanism of the receptor function.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
