The role of thymic nurse cells in T cell development.
Item
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Title
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The role of thymic nurse cells in T cell development.
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Identifier
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AAI9605619
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identifier
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9605619
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Creator
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Li, Yang.
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Contributor
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Adviser: Jerry Guyden
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Cell | Health Sciences, Immunology
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Abstract
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SV40 immortalized thymic nurse cells (TNCs) were shown to maintain the ability to selectively internalize a subpopulation of {dollar}\alpha\beta{dollar} {dollar}\rm TCR\sp+CD4\sp+CD8\sp+{dollar} thymocytes in vitro. We have now developed temperature sensitive lines of TNCs using the SV40 viral mutant tsA58. One temperature sensitive line of TNCs, tsTNC-1, was shown to be able to rescue a subset of {dollar}\rm CD4\sp+CD8\sp+{dollar} thymocytes from programmed cell death at 32{dollar}\sp\circ{dollar}C, the temperature at which binding and internalization were detected. Rescue was not detected at 38{dollar}\sp\circ{dollar}C, the temperature at which thymocyte binding was undetectable. The rescued population of thymocytes showed a reduced level of apoptosis as measured by the DNA fragmentation assay. TNC rescue resulted in a shift of {dollar}\rm CD4\sp+CD8\sp+{dollar} thymocytes from immature {dollar}\rm TCR\sp{lcub}low{rcub}{dollar} cells to the more mature {dollar}\rm TCR\sp{lcub}int{rcub}{dollar} phenotype. The rescue activity of tsTNC-1 cells at 32{dollar}\sp\circ{dollar}C was blocked with the addition of antibodies to either class I or class II MHC antigens. These results suggest that TNC rescue is facilitated through an interaction between the TCR and the MHC, and that the resulting survivors mature to a phenotype within the double positive stage of development that is indicative of positive selection.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
